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Microbial screening in hydroxylation of l-proline
Authors:M-C Bontoux
Institution:Rhodia—Centre de Recherches et Technologies de Lyon, BP 62, 85 rue des frères Perret, F-69192 Saint Fons Cedex, France
Abstract:Microbial screening of 250 wild type strains resulted in identification of five strains with the activity of prolyl hydroxylase. All five strains hydroxylated regioselectively and enantioselectively l-proline into 4(R)-trans-hydroxy-l-proline 1. The best conversions were obtained with a wild type of Aeromonas caviae. 3-Hydroxylase activity was not detected.
Keywords:l-Proline" target="_blank">l-Proline  Hydroxylation  Microbial screening
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