| Abstract: | A micro high-performance liquid chromatographic (micro-HPLC) method was developed for the determination of purpurea glycoside A, purpurea glycoside B, and glucogitaloxin in Digitalis purpurea leaves. The extract of dry leaf powder was applied to a Sep-Pak silica cartridge followed by preparative thin-layer chromatography prior to micro-HPLC analysis. The analysis was performed on an ODS micro column, using acetonitrile-methanol-water (21:20:45) and ultraviolet detection (220 nm). The amounts of purpurea glycoside A, purpurea glycoside B, and glucogitaloxin per 100 mg of dry leaf powder were estimated to be 52.6, 50.7, and 108.6 μg, respectively. |
