Preparation,Characterization and Anticoagulant Activity of Guar Gum Sulphate |
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Abstract: | Guar gum was chemically modified by sulphonation using chlorosulphonic acid (ClSO3H) as a reagent. Effects of molar ratio of ClSO3H to glucopyranosic unit (ClSO3H/GU), reaction time and reaction temperature on the degree of sulphonation (DS) and molecular weight (Mw) of products were studied. The structures of guar gum sulphate were investigated by GPC, FT‐IR and UV‐Visible spectroscopy. Activated partial thromboplastin time (APTT) assay showed that the guar gum sulphate could inhibit the intrinsic coagulant pathway. The anticoagulant activity strongly depended on the DS and Mw of polysaccharides. DS>0.56 was essential for anticoagulant activity. The guar gum sulphate with the DS of 0.85 and the Mw of 3.40×104 had the best blood anticoagulant activity. |
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Keywords: | guar gum sulphonation anticoagulant activity |
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