EFFICIENT FLUORESCEIN SPIROLACTAM AND BIS-SPIROLACTAM SYNTHESIS

Reaction of fluorescein methyl ester at ambient temperature with a variety of amines containing different functional groups afforded a family of spirolactams, the structures of which were verified by MS, NMR, and x-ray crystallography. Bis-spirolactams were prepared by subsequent reaction of an aminospirolactam with another molecule of fluorescein methyl ester.