Use of focus stacking and SfM techniques in the process of registration of a small object hologram

In this Letter, a method for shape visualization of small objects(microscopic) in the form of a hologram is presented. It consists of a standard optical set-up for small object registration(i.e., stereoscopic or biological microscope). The focus stacking technique is used to obtain a series of images with increased depth of field and on them a shape reconstruction procedure(structure from motion, SfM) is made. With use of a dense cloud of points, a sequence of parallax-related images suitable for Geola's digital holographic printing is generated. The holographic printer produces single-parallax holographic(full three-dimensional) images of real or virtual objects.