雷公藤多苷致大鼠精子畸形初步研究

目的 采用雷公藤(Tripterygium wilfordii)多苷灌胃造模,分析大鼠精子异常形态,详细描述大鼠精子异常形态类型,为研究大鼠精子畸形提供参考.方法 雄性成年SD大鼠,雷公藤多苷剂量为30 mg/kg灌胃,给药4周及8周后,分别取大鼠附睾精子,用Diff-Quik染色法,在光镜下行精子形态分析.结果 (1...

A Preliminary Study on the Sperm Deformity of Rats Caused byTripterygium Wilfordii Polyglycosides

Abstract:ObjectiveUsingTripterygium wilfordiipolyglycoside to make gastric gavage, to analyze and describe the abnormal sperm morphology of rats, and to provide a reference to the relevant study.MethodMale adult SD rats were selected andT. wilfordiipolyglycosides 30 mg / ( kg· d) was intragastrically administered. After 4 and 8 weeks of administration, the epididymal sperm of the rats were taken respectively. After Diff-Quik staining, sperm morphology analyzed under light microscope.Result(1) Using the Diff-Quik staining method to stain rats sperm, it was simple, fast, well colored and clear. ( 2) Rat sperm is clearly distinguishable from human sperm, such as ①The normal rate of sperm morphology was high and the deformity rate was low. ②The head of the rat sperm was curved and sickle-shaped, and similar to surgical sutures with needles. ③ The main segment of rat sperms was significantly longer than human. (3) Deformed sperm morphology ofT. wilfordiimodel rats were no hook, folded head, small head, no head, neck fracture, no tail, tail curl and tail fracture,and the most common types were headless and tailless. (4) The rat sperm deformity rate increased after gavage ofT. wilfordiipolyglycoside tablets after 4 weeks, and compared with the blank group, the difference was statistically significant (P= 0. 00 < 0. 01) . Compared with the blank and the 4-week groups, the deformity rate of rats in the 8 - week group increased, the differences was statistically significant (P= 0. 00<0. 01) .Conclusion( 1) Diff-Quik staining could be used as a staining method for rat sperms. (2) Rat sperm of rats or human were quite different. ( 3) Gavage ofT. wilfordiipolyglycoside could cause various types of sperm deformities in the head, middle and main segments of rat sperms, and the sperm deformation rate was significantly increased. It could be used as an animal model of teratozoospermia.