A Long-wavelength Emissive Phenothiazine Derived Fluorescent Probe for Detecting HOCl Upregulation in 5-FU Stimulated Living Cells |
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| Authors: | WANG Bingya GUO Xiaomei LIU Zuodong WU Yongquan HOU Ji-Ting |
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| Institution: | 1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, P. R. China;2. School of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou 341000, P. R. China;3. Henan Key Laboratory of Utilization of Non-metallic Mineral in the South of Henan, Xinyang Normal University, Xinyang 464000, P. R. China |
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| Abstract: | The endogenous hypochlorous acid(HOCl) has been evidenced in a variety of cellular courses. However, the role of HOCl in most pathophysiological processes still keeps unclear because of the limited detecting tools. In this work, we presented the pre- paration of a phenothiazine-derived fluorescent probe ClO-1 for HOCl detection with a cyanopyridinium moiety to improve its water solubility and lengthen its emission wavelength. The HOCl-promoted oxidation of sulfur atom in the probe resulted in a 460-fold emission intensity enhancement at 635 nm with high selectivity and sensitivity(detection limit: 1.12 nmol/L). The rapid response(5 s) also endowed the probe with real-time detection ability. Successfully, ClO-1 was devoted to the bioimaging of endogenous HOCl in inflamed RAW 264.7 cells and 5-fluorouracil-treated MCF-7 cells. |
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| Keywords: | Fluorescent probe Phenothiazine Hypochlorous acid 5-Fluorouracil Bioimaging |
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