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Application of derivative spectrophotometry to determination of flavonoid mixtures
Authors:Baranowska I  Raróg D
Affiliation:Department of Analytical and General Chemistry, Silesian Technical University, M. Strzody St. 9, 44-100 Gliwice, Poland.
Abstract:Derivative spectrophotometry (DS) was applied to determination of flavonols and a flavon in two-compound mixtures by means of 'zero-crossing' technique. Selected mixtures were, quercetin-kaempferol, quercetin-myricetin and quercetin-luteolin. The developed method was used as a complementary technique to a HPLC system, which does not separate quercetin and luteolin.
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