Application of a microbial sensor to the quality control of meat freshness

Isosorbide dinitrate (ISDN) is reduced voltammetrically at gold working electrode surface in aqueous sodium sulfite solution and produces a voltammogram with its peak current proportional to the concentration of ISDN in the range of 1.3-2340 mug ml(-1). Sodium sulfite is used as supporting electrolyte and oxygen removing agent and, therefore, no nitrogen gas purging for elimination of soluble oxygen in, the sample solution is required. The limit of detection (LOD) of the method is 0.0838 mug ml(-1) and the relative standard deviation (R.S.D.) of 24 replicate determination of 52 mug ml(-1) of ISDN is 3.60%. The method is used for quantitative analysis of ISDN in arterial plasma, synthetic serum and pharmaceutical dosage form. The results are compared with those obtained from GC determination method. The method is sensitive and requires little sample preparation in a wide concentration range.