Use of amino-carbonyl reaction and chemiluminescence detection to the flow-injection determination of some amino acids |
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Authors: | Michałowski J Kojło A |
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Affiliation: | Institute of Chemistry, University of Bia?ystok, al. J. Pi?sudskiego 11/4, 15-443 Bia?ystok, Poland. |
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Abstract: | Some amino acids were found to react with carbonyl functional groups of humic acid. Products of this reaction give strong chemiluminescence during their oxidation with N-bromosuccinimide (NBS) in alkaline solution. Humic acids from different sources produced similiar signal magnitude. This effect was employed to the flow-injection determination of glycine and arginine in pharmaceutical formulations with considerable selectivity against different amino compounds. The proposed method is fast and simple. Detection limit is 0.20 and 0.25 mg.l(-1) for glycine and arginine respectively, and 115 samples per h can be determined. |
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