Flow injection spectrophotometric determination of reducing sugars using a focalized coiled reactor in a domestic microwave oven |
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Authors: | Oliveira A F Fatibello-Filho O |
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Institution: | 1. Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3. Jinling College, Nanjing Normal University, Nanjing 210097, China |
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Abstract: | A flow injection (FI) spectrophotometric procedure for determining reducing sugars content in sugar cane juices using a focalized PTFE coiled reactor positioned at the output antenna of a domestic microwave oven at 700 W is proposed. In this system, sample solution converge to 1.0 mol l(-1) NaOH and 5.2 mmol l(-1) K(3)Fe(CN)(6) solutions previously mixed and the decrease of hexacyanoferrate(III) concentration was monitored at 420 nm. Under best analytical conditions, there was a direct relationship between absorbance decrease and reducing sugar content (fructose plus glucose concentrations) in the concentration range from 50 to 1200 micromol l(-1) with a detection limit of 15 micromol l(-1). The relative standard deviations (rds) were less than 1.4% for ten injection of 400 and 800 micromol l(-1) fructose solution and the analytical frequency was 70 h(-1). A paired t-test showed that all results obtained for sugar cane juices using this FI procedure and the Somogyi-Nelson batch procedure agree at the 95% confidence level. |
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