赤霉素类植物激素分析方法研究进展

赤霉素(gibberellins, GAs)作为植物体内广泛存在的一类激素,可刺激植物叶和芽的生长,对植物的生长起着至关重要的调控作用。截至目前,已发现的GAs高达136种之多。所有已知的GAs均为二萜酸,具有相似的化学结构,都含有赤霉素烷骨架,即4个环,仅环上双键、羟基数目和位置不同。但由于GAs缺乏特定光学特性(紫外和荧光)和化学特性,同时在植物体内含量极低(一般为ng/g,甚至为pg/g水平),常规的分析方法和检测技术难以实现对该类物质的定性定量分析。近年来相关研究人员一直致力于各种高效、快速、灵敏的GAs分析新方法和新技术的研究。本文就GAs的检测方法和分析技术进行综述,为更好地开展赤霉素类植物激素的研究提供参考。

Recent advances in the analysis of gibberellins plant hormones

Gibberellins (GAs) are a class of phytohormones that exert profound and diverse effects on plant growth and development, such as seed germination and leaf expansion. Up to now, 136 members of GAs have been identified and recognized. All known GAs are diterpenoid acids with similar chemical structures, only double bonds, hydroxyl numbers and locations on gibberellin alkane skeleton are different. However, the content of GAs in plants is of ultra trace levels (usually at ng/g and even pg/g levels) with little ultraviolet (UV) absorption, no fluorescence and no distinguishing chemical characteristics. Moreover, the matrix of plant samples is complicated. Thus, quantification of GAs is always extremely difficult. Nowadays, the bottle necks for the study of GAs in plants are due to the lack of efficient sample preparation and sensitive detection techniques. This article reviews the analytical methods for determination of GAs in recent years, hoping to provide some references to develop new methods and techniques.