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High-performance liquid chromatographic analysis of anthraquinone compounds in the Laurera benguelensis
Authors:N Manojlovi?  Z Markovi?  W Gritsanapan and K Boonpragob
Institution:(1) Department of Pharmacy, Medical Faculty, University of Kragujevac, S. Markovića 69, 34000 Kragujevac, Serbia;(2) Department of Biochemical and Medical Sciences, State University of Novi Pazar, Vuka Karadžića bb, Novi Pazar, 36300, Serbia;(3) Faculty of Pharmacy, Mahidol University, 10040 Bangkok, Thailand;(4) Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand
Abstract:A high-performance liquid chromatographic (HPLC) method has been developed for the characterization of anthraquinone metabolites in extracts of the lichen Laurera benguelensis. With this method four anthraquinone derivatives 1,8-dihydroxy-3-methoxy-6-methylanthraquinone, 1,8-dihydroxy-3-formyl-6-methoxyanthraquinone, 1,8-dihydroxy-3-hydroxymethyl-6-methoxy-anthraquinone and 1,3,8-trihyroxy-6-methylanthraquinone can be analyzed. Components of lichen were detected by characteristic ultraviolet spectra and relative retention times. This is first report of phytochemical analysis of L. benguelensis. Importance of this research is in recognizing some new source (lichen and its extracts) as a natural emplacement of antioxidants because oxidation with free radicals or autooxidation is big problem for preservation of food products. The article is published in the original.
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