| 低浓度CTAB对血红蛋白与阿昔洛韦间相互作用与性质的影响 |
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| 引用本文： | 刘天晴,郭荣. 低浓度CTAB对血红蛋白与阿昔洛韦间相互作用与性质的影响[J]. 中国化学, 2006, 24(5): 620-626. DOI: 10.1002/cjoc.200690119 |
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| 作者姓名： | 刘天晴 郭荣 |
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| 作者单位： | [1]School of Chemistry and Chemical Engineering, Nanjing University, Jiangsu 210093, China [2]School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 250002, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 20573091, 20233010）. |
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| 摘 要： | The effects of cetyltrimethylammonium bromide （CTAB） on the properties of hemoglobin （Hb） at low CTAB concentration were studied in Hb/acyclovir/CTAB system by the methods of UV-Vis spectrum, fluorescence, zeta potential, conductivity and negative-staining transmission electron microscope （TEM）. With the increase of CTAB concentration, the UV peak intensity at 276 nm, the intrinsic fluorescence, the zeta potential of Hb and the system conductivity were all enhanced. Hb was easily oxidized to oxyHb and hemichrome. In Hb/acyclovir/CTAB system, CTAB made the UV-Vis spectrum, fluorescence, conductivity and conformation of Hb tend to be returned to those of the original Hb but the zeta potential not to do so. The UV absorption peak of Hb-acyclovir complex disappeared and the tight structure of Hb aroused by acyclovir was refolded. When CTAB concentration was higher than 5 × 10 ^5 mol/L, the two absorption peaks at 536 and 576 nm appeared again, and the Hb structure became looser again.
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| 关 键 词： | 血红蛋白 鲸蜡基二甲基溴化铵 嘌呤核苷类似物 电子显微镜分析 相互作用 性质分析 |
| 收稿时间： | 2005-08-22 |
| 修稿时间： | 2005-08-222006-01-11 |
Influence of Low Cetyltrimethylammonium Bromide Concentration on the Interactions and Properties of Hemoglobin with Acyclovir |
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| LIU Tian-Qing,GUO Rong. Influence of Low Cetyltrimethylammonium Bromide Concentration on the Interactions and Properties of Hemoglobin with Acyclovir[J]. Chinese Journal of Chemistry, 2006, 24(5): 620-626. DOI: 10.1002/cjoc.200690119 |
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| Authors: | LIU Tian-Qing GUO Rong |
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| Abstract: | The effects of cetyltrimethylammonium bromide (CTAB) on the properties of hemoglobin (Hb) at low CTAB concentration were studied in Hb/acyclovir/CTAB system by the methods of UV‐Vis spectrum, fluorescence, zeta potential, conductivity and negative‐staining transmission electron microscope (TEM). With the increase of CTAB concentration, the UV peak intensity at 276 nm, the intrinsic fluorescence, the zeta potential of Hb and the system conductivity were all enhanced. Hb was easily oxidized to oxyHb and hemichrome. In Hb/acyclovir/CTAB system, CTAB made the UV‐Vis spectrum, fluorescence, conductivity and conformation of Hb tend to be returned to those of the original Hb but the zeta potential not to do so. The UV absorption peak of Hb‐acyclovir complex disappeared, and the tight structure of Hb aroused by acyclovir was refolded. When CTAB concentration was higher than 5×10?5 mol/L, the two absorption peaks at 536 and 576 nm appeared again, and the Hb structure became looser again. |
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| Keywords: | hemoglobin cetyltrimethylammonium bromide acyclovir property interaction |
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