基于纳米自组装技术的功能化溶胶-凝胶过氧化氢生物传感器

结合功能化溶胶-凝胶(sol-gel)网络结构、自组装技术和纳米粒子效应,提出一种生物传感界面构建方法.利用自组装技术在玻碳电极表面组装氨基化sol-gel膜,通过与自组装膜间的强烈作用将纳米金粒子固定于sol-gel网络中,再通过静电吸附作用实现辣根过氧化物酶(HRP)在纳米金粒子表面的固定化,构建纳米自组装HRP传感界面.将制备的传感器用于对H2O2的催化还原,很好地保持了酶的生物活性,改善了传感器的灵敏度.

H_2O_2 biosensor based on functional sol-gel network and nanoparticle self-assemble techniques

A novel immobilization method of horseradish peroxidase applied to a biosensor for detecting hydrogen per- oxide has been proposed by incorporating functional sol-gel film and nanoparticle self-assemble techniques.The inter- facial design of biosensor was prepared by self-assembling the amino-containing sol-gel onto the glass carbon electrode which surface present high concentration of reactive hydroxyl/oxide groups,then chemisorbing gold nanoparticles into the amino-containing sol-gel networks,and finally ...