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高效液相法测定胃康片中呋喃唑酮、甘珀酸钠和盐酸小檗碱
引用本文:张英.高效液相法测定胃康片中呋喃唑酮、甘珀酸钠和盐酸小檗碱[J].色谱,2002,20(4):350-352.
作者姓名:张英
作者单位:深圳职业技术学院生物应用工程系,广东,深圳,518055
摘    要: 用高效液相 (HPLC)梯度洗脱法同时测定复方胃康片中呋喃唑酮、甘珀酸钠和盐酸小檗含量。使用No va PakC18柱 ,以乙腈和 0 0 2mol/L磷酸盐缓冲溶液 (pH 7 0 )为流动相 ,3,5 二硝基苯甲酸作为内标溶液 ,使用UV检测器 ,在 2 5 4nm处对样品中上述 3种成分进行测定。测定结果表明 ,呋喃唑酮在 14 1 2mg/L~ 12 70 8mg/L、甘珀酸钠在 10 0 6mg/L~ 90 5 4mg/L、盐酸小檗碱在 99 2mg/L~ 892 8mg/L时其相对峰面积 (标准品与内标的峰面积之比 )与进样质量浓度有良好的线性关系 ,其线性相关系数分别为r =0 9997,r =0 9995和r =0 9991。

关 键 词:高效液相法  复方胃康片剂  呋喃唑酮  甘珀酸钠  盐酸小檗碱
文章编号:1000-8713(2002)04-0350-03
修稿时间:2002年3月4日

Determination of Furazolidone, Carbenoxolone Sodium and Berberine Hydrochloride in Wei Kang Tablets by Reversed-Phase High Performance Liquid Chromatography(RP-HPLC)
ZHANG,Ying.Determination of Furazolidone, Carbenoxolone Sodium and Berberine Hydrochloride in Wei Kang Tablets by Reversed-Phase High Performance Liquid Chromatography(RP-HPLC)[J].Chinese Journal of Chromatography,2002,20(4):350-352.
Authors:ZHANG  Ying
Institution:Applied Biological Engineering Department, Shenzhen Polytechnic, Shenzhen 518055, China. zhangying999@163.net
Abstract:An HPLC method with gradient elution was developed to separate and determine furazolidone, carbenoxolone sodium and berberine hydrochloride in Wei Kang Tablets on Nova-Pak C18 column (150 mm x 3.9 mm i.d., 4 microns), using acetonitrile-phosphate buffer solution (pH 7.0) as mobile phase and 3,5-dinitrobenzoic acid as the internal standard. Detection was performed with UV detector at 254 nm. The calibration curves were linear within the ranges of 141.2 mg/L -1,270.8 mg/L for furazolidone(r = 0.9997), 100.6 mg/L -905.4 mg/L for carbenoxolone sodium(r = 0.9995) and 99.2 mg/L -892.8 mg/L for berberine hydrochloride(r = 0.9991). The recoveries and RSDs were 99.5% and 1.38% for furazolidone, 100.3% and 1.73% for carbenoxolone sodium, 97.3% and 1.97% for berberine hydrochloride respectively. The results show that this method is simple, rapid, specific, accurate and reproducible.
Keywords:high performance liquid chromatography  Wei Kang Tablets  furazolidone  carbenoxolone sodium  berberine hydrochloride
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