Antibody development to testosterone and its application in capillary electrophoresis-based immunoassay |
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| Authors: | Chen Hong-Xu Zhang Xin-Xiang |
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| Affiliation: | Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry, Peking University, Beijing, P. R. China. |
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| Abstract: | The present report describes a rapid, simple, and highly selective approach to perform testosterone competitive immunoassay by CE and LIF detection. The method involves using synthesized fluorescence-labeled testosterone as a tracer to compete with testosterone. Two polyclonal antibodies (antibody (Ab) arised from T-3-BSA (Ab(3)) and Ab arised from T-17-BSA (Ab(17))) and their respective tracers have been optimized and Ab(3) system is used for the quantification of testosterone by CE-based immunoassay. The method is developed with a wide working range of 3.70-2000 ng/mL and an LOD at 1.11 ng/mL. Tests for normal and positive urine samples show that this method has the potential to be applied in testosterone doping control. |
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