Some pitfalls in PAGE-LA-ICP-MS for quantitative elemental speciation of dissolved organic matter and metalomics |
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Authors: | Maria S Jiménez Maria T Gomez L Rodriguez L Martinez Juan R Castillo |
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Institution: | (1) Analytical Spectroscopy and Sensors Group, Institute of Environmental Sciences, University of Saragossa, Pedro Cerbuna 12, Saragossa, 50009, Spain |
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Abstract: | An experimental approach to evaluate the capabilities and limitations of polyacrylamide gel electrophoresis (PAGE) and laser
ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for quantitative elemental speciation is presented. Two
metalloproteins (superoxide dismutase, containing Cu and Zn, and thyroglobulin, containing I) with high binding affinity for
metals, and metal-dissolved organic matter (DOM) complexes (from a compost leachate sample) which show different types of
metal binding are studied. Iodine can be quantitatively detected in thyroglobulin after PAGE-LA-ICP-MS using either sodium
dodecyl sulfate (SDS) PAGE or native PAGE. However, detection of Cu and Zn in superoxide dismutase after PAGE-LA-ICP-MS depends
on the conditions of the PAGE method because possible metal losses can occur (either with SDS-PAGE or with native PAGE). The
use of PAGE-LA-ICP-MS to study the contribution of DOM to the mobilization of metals from environmental samples is possible,
but it depends also on the PAGE separation conditions owing to disequilibrium effects of metal-DOM complexes.
Presented at the 4th International Conference on Trace Element Speciation in Biomedical, Nutritional and Environmental Sciences,
25–29 May 2008, Munich-Neuherberg, Germany. |
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Keywords: | Gel electrophoresis Laser ablation inductively coupled plasma mass spectrometry Elemental speciation Metal-binding proteins Metalloproteins Metal binding to natural organic matter |
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