Characterization of temperature dependent modifier effects in SFC using linear solvation energy relationships |
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Authors: | J A Blackwell R W Stringham |
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Institution: | (1) Chemical Process Research and Development, Dupont Merck Pharmaceutical Company, Chambers Works, PRF-1 (S-1), 08023 Deepwater, NJ, USA |
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Abstract: | Summary Linear solvation energy relationships (LSERs) are used to probe the changes in mobile and stationary phase properties of a
carbon dioxide-based mobile phase and a polymeric stationary phase under near-critical conditions. Four mobile phase modifiers
are compared with respect to dipolarity/polarizability, hydrogen bond donating and accepting ability, and other intermolecular
interactions as a function of temperature. As temperature nears the mixture critical point, the differences in these properties
between the mobile and stationary phases change to reflect the growing heterogeneity in mobile phase component distribution
at the chromatographic interface. The stationary phase loses many of its original characteristics and takes on characteristics
typical of the mobile phase modifier due to preferential adsorption of the modifier at the surface of the stationary phase. |
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Keywords: | Supercritical fluid chromatography Packed columns Selectivity relationships Hydrogen bonding Linear solvation energy relationships |
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