| 毛细管柱固定蛋白质方法的研究进展 |
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| 引用本文： | 陈文静,凌笑梅. 毛细管柱固定蛋白质方法的研究进展[J]. 分析化学, 2009, 37(6) |
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| 作者姓名： | 陈文静 凌笑梅 |
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| 作者单位： | 北京大学药学院药物分析教研室,北京,100083;北京大学药学院药物分析教研室,北京,100083 |
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| 摘 要： | 在熔融石英毛细管中,固定化的蛋白质(酶)在手性拆分、蛋白质肽谱、药物筛选、样品预浓缩等方面具有重要应用.本文对用于毛细管中蛋白质固定的溶胶-凝胶法、物理吸附法、离子鳌合吸附法、基于脂质的蛋白固定和共价键合法进行了综述.
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| 关 键 词： | 蛋白质固定 溶胶-凝胶法 物理吸附法 离子鳌合吸附法 脂质固定蛋白 共价键合 评述 |
Advance of Methods of Protein Immobilization on Capillary Column |
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| CHEN Wen-Jing,LING Xiao-Mei. Advance of Methods of Protein Immobilization on Capillary Column[J]. Chinese Journal of Analytical Chemistry, 2009, 37(6) |
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| Authors: | CHEN Wen-Jing LING Xiao-Mei |
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| Affiliation: | Department of Pharmaceutical Analysis;School of Pharmaceutical Science;Peking University;Beijing 100083 |
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| Abstract: | In fused silica capillary the immobilized proteins(enzymes) have important applications in chiral separation,peptide mapping,drug screening,preconcentration and so on.In this article,we reviewed various methods for protein immobilization in capillary,including sol-gel method,physical adsorption,ion chelate adsorption,lipid-based protein immobilization and covalently bound. |
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| Keywords: | Protein immobilization sol-gel physical adsorption ion chelate adsorption lipid-based protein immobilization covalently bound review |
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