Indirect fluorimetric detection of proteins via postcolumn mixing with fluorescence probe in size-exclusion chromatography |
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| Authors: | T Takeuchi K Kawai Y Kitamaki T Miwa |
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| Institution: | (1) Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, 501-1193 Gifu, Japan |
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| Abstract: | Summary Proteins were visualized by postcolumn mixing with 2-p-toluidinyl-6-naphthalene sulfonate or 1-anilino-8-naphthalene sulfonate in size-exclusion chromatography. The indirect detection
is based on fluorescence enhancement of the fluorescence probe owing to hydrophobic interaction with proteins. Bovine serum
albumin gave the highest signal intensity among the proteins examined. |
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| Keywords: | Column liquid chromatography Size-exclusion chromatography Indirect fluorimetric detection Proteins Fluorescence probe |
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