Immobilization of proteolytic enzymes trypsin and α-chymotrypsin to cellulose matrix |
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| Authors: | N. E. Kotel’nikova S. A. Mikhailova E. N. Vlasova |
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| Affiliation: | (1) Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia |
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| Abstract: | Trypsin and α-chymotrypsin were immobilized on a microcrystalline cellulose matrix by adsorption interaction with cellulose. The adsorption of enzymes was determined as a function of their concentration and the pH of solutions. Mechanisms of enzyme binding to functional groups of cellulose were suggested. |
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