High-sensitive protein analysis by FESI-CE-MALDI-MS |
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Authors: | Pourhaghighi Mohammad Reza Busnel Jean-Marc Girault Hubert H |
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Institution: | Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. |
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Abstract: | Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iontophoretic fraction collection interface for the coupling of CE and MALDI-MS, the potential of the combination of both techniques for the analysis of intact proteins is assessed. To further provide a bioanalytical platform with high-sensitivity capabilities, field-enhanced sample injection is integrated as on online preconcentration strategy upstream from the electrokinetic separation. Under optimized conditions, more than 3200- and 4800-fold improvement, respectively in terms of peak height and peak area, as well as LODs ranging from 5 to 10 nM, has been achieved. |
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Keywords: | CE Field‐enhanced sample injection Matrix‐assisted laser desorption/ionization mass spectrometry On‐line preconcentration |
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