核磁共振技术测试不同性别大鼠尿液代谢物的研究

采用核磁共振波谱技术测试不同性别大鼠尿液代谢物,分析性别因素对大鼠尿液代谢成分的影响.大鼠尿液核磁共振氢谱（1HNMR谱）结果采用主成分分析（principal component analysis,PCA）和正交偏最小二乘判别分析（orthogonal to partial least squares discriminant analysis,OPLS-DA）方法分析,得到不同性别大鼠尿液中的差异性代谢物.PCA分析结果显示2组尿液代谢成分有明显的差异,进一步进行OPLS-DA分析可以判别出2组尿液中具有差异性的代谢物.结果显示,雌性大鼠尿液中的丙氨酸、缬氨酸、鸟氨酸等氨基酸类以及乙酸、硫胺、氨基马尿酸、苯乙胺、氧氨嘧啶等代谢物含量高于雄性大鼠,差异有统计学意义（p〈0.05）.雄性大鼠尿液中的甲胺、二甲胺、三甲胺、肌酸酐、尿囊素、延胡索酸、甲酸等代谢物则明显高于雌性大鼠,差异有统计学意义（p〈0.05）.性别因素对大鼠尿液中的代谢成分有一定的影响.

Metabonomic Analysis of Rat Urine Using Nuclear Magnetic Resonance Spectroscopy

The metabonomic groups of male and female rat urine have been investigated by nuclear magnetic resonance (NMR) spectroscopy. Two pattern recognition techniques including the principal component analysis (PCA) and the orthogonal to partial least squares discriminant analysis (OPLS-DA) were used to find the difference of metabolic groups between male and female rats, respectively. The results of PCA and OPLS-DA analyses indicated that the amounts of valine, alanine, acetate, thiamine, ornithine, aminohippurate, phenylethylamine and cytosine in the urine samples of female rats were obviously higher than in those of male rats, based on the sufficient statistical variation (p<0.05). On the other hand, the amounts of methylamine, dimethylamine, trimethylamine, creatinine, allantoin, fumarate, formate in the urine samples of male rats were higher than those of female rats (p<0.05). The results showed that the gender of rat has certain effects on the metabolites of rat urine.