| Study on the violet LED-induced fluorescence spectra of thioredoxin reductase from human brain |
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| 引用本文： | 兰秀风,杨涛,高淑梅,骆晓森,沈中华,陆建,倪晓武,许琳. Study on the violet LED-induced fluorescence spectra of thioredoxin reductase from human brain[J]. 中国光学快报(英文版), 2003, 1(3) |
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| 作者姓名： | 兰秀风 杨涛 高淑梅 骆晓森 沈中华 陆建 倪晓武 许琳 |
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| 作者单位： | Department of Applied Physics，Nanjing University of Science & Technology，Nanjing 210094，Department of Biochemistry，Nanijng Medical College，Second Military Medical University，Nanjing 210099，Department of Applied Physics，Nanjing University of Science & Technology，Nanjing 210094，Department of Biochemistry，Nanijng Medical College，Second Military Medical University，Nanjing 210099，Department of Applied Physics，Nanjing University of Science & Technology，Nanjing 210094，Department of Applied Physics，Nanjing University of Science & Technology，Nanjing 210094，Department of Applied Physics，Nanjing University of Science & Technology，Nanjing 210094，Department of Biochemistry，Nanjing Medical College，Second Military Medical University，Nanjing 210099 |
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| 摘 要： | The technique of fluorescence spectroscopy is applied to study thioredoxin reductase (TrxR) in the cells of human brain. Experimental results show that, by the violet light emitting diode (LED, Amax=407 nm) light irradiation, TrxR is able to emit three striking spectral bands (528 - 582 nm; 588 - 660 nm; 683 - 700 nm). The fluorescence intensity is linear to the concentration of TrxR. The spectrum of denatured TrxR is rather different from that of organized TrxR, which reflects the structure change between denatured TrxR and organized TrxR. Furthermore, physical and biochemical mechanisms of fluorescence production for LED light-induced TrxR spectra and its characteristics are analyzed. This paper may be useful to better understand the structure of TrxR, and to provide new spectroscopic information to improve the resolution for this kind of biology structure.
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Study on the violet LED-induced fluorescence spectra of thioredoxin reductase from human brain |
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| Abstract: | The technique of fluorescence spectroscopy is applied to study thioredoxin reductase (TrxR) in the ce;lsof human brain. Experimental results show that, by the violet light emitting diode (LED, λmax=407 nm)light irradiation, TrxR is able to emit three striking spectral bands (528 - 582 nm; 588 - 660 nm; 683 - 700nm). The fluorescence intensity is linear to the concentration of TrxR. The spectrum of denatured TrxR israther different from that of organized TrxR, which reflects the structure change between denatured TrxRand organized TrxR. Furthermore, physical and biochemical mechanisms of fluorescence production forLED light-induced TrxR spectra and its characteristics are analyzed. This paper may be useful to betterunderstand the structure of TrxR, and to provide new spectroscopic information to improve the resolutionfor this kind of biology structure. |
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