| Abstract: | With p‐thiocresol as internal standard, quantitative analysis of captopril, a synthetic angiotensin converting enzyme inhibitor, was achieved by the combination of the multiplicative effects model with surface‐enhanced Raman spectroscopy (SERS). The multiplicative effects model was adopted to correct the detrimental effects caused by the heterogeneity in the physical properties of enhancing substrate (i.e. Ag nano‐particles). Experimental results showed that the calibration model built on the SERS spectra of the calibration captopril samples prepared with ultrapure water could attain quite satisfactory concentration predictions for captopril in both real‐world tablet samples and plasma samples. The recovery rates were in the range of 94.3% to 109.8%, which were in substantial agreement with the corresponding results of LC‐MS/MS. The limit of detection and limit of quantification were estimated to be 0.149 and 0.451 μM, respectively. The proposed approach has advantages of relatively low cost, simplicity, high sensitivity and good accuracy and therefore can be further developed and extended to a routine method for the quantification of captopril in complex systems. Copyright © 2015 John Wiley & Sons, Ltd. |
