Size selective sampling using mobile, 3D nanoporous membranes |
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| Authors: | Christina L Randall Aubri Gillespie Siddarth Singh Timothy G Leong David H Gracias |
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| Institution: | (1) Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA;(2) Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA;(3) Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA |
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| Abstract: | We describe the fabrication of 3D membranes with precisely patterned surface nanoporosity and their utilization in size selective
sampling. The membranes were self-assembled as porous cubes from lithographically fabricated 2D templates (Leong et al., Langmuir 23:8747–8751, 2007) with face dimensions of 200 μm, volumes of 8 nL, and monodisperse pores ranging in size from approximately 10 μm to 100 nm.
As opposed to conventional sampling and filtration schemes where fluid is moved across a static membrane, we demonstrate sampling
by instead moving the 3D nanoporous membrane through the fluid. This new scheme allows for straightforward sampling in small
volumes, with little to no loss. Membranes with five porous faces and one open face were moved through fluids to sample and
retain nanoscale beads and cells based on pore size. Additionally, cells retained within the membranes were subsequently cultured
and multiplied using standard cell culture protocols upon retrieval.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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| Keywords: | Microfluidics/Microfabrication Nanoparticles/Nanotechnology Separations/Instrumentation Bioanalytical methods |
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