| 高效液相色谱-基质辅助激光解吸电离串联飞行时间质谱分离鉴定牛乳铁蛋白素 |
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| 引用本文： | 安美忱,刘宁. 高效液相色谱-基质辅助激光解吸电离串联飞行时间质谱分离鉴定牛乳铁蛋白素[J]. 色谱, 2010, 28(2): 180-184 |
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| 作者姓名： | 安美忱 刘宁 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室, 黑龙江 哈尔滨 150030 |
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| 基金项目： | 东北农业大学创新团队基金项目 |
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| 摘 要： | 建立了高效液相色谱-基质辅助激光解吸电离串联飞行时间质谱(MALDI-TOF/TOF MS)分离鉴定牛乳铁蛋白素(bovine lactoferricin, LfcinB)的方法。采用胃蛋白酶酶解牛乳铁蛋白,酶解液离心后取上清液,经过离子交换色谱、反相液相色谱、透析等技术分离,对分离得到的目标产物进行抗菌活性分析、蛋白含量测定和MALDI-TOF/TOF MS鉴定。分离得到高活性的LfcinB,其相对分子质量为3124.89,蛋白含量为18.20 μg/mL。本方法具有精度高、分析速度快和分辨能力强等优点,是其他传统的分析鉴定LfcinB方法所无法比拟的,为进一步研究LfcinB奠定了基础。
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| 关 键 词： | 高效液相色谱 基质辅助激光解吸电离串联飞行时间质谱 牛乳铁蛋白 牛乳铁蛋白素 |
| 收稿时间： | 2009-06-30 |
| 修稿时间： | 2009-08-30 |
Separation and identification of bovine lactoferricin by high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry |
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| AN Meichen,LIU Ning. Separation and identification of bovine lactoferricin by high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry[J]. Chinese journal of chromatography, 2010, 28(2): 180-184 |
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| Authors: | AN Meichen LIU Ning |
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| Affiliation: | Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | A high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry(HPLC-MALDI-TOF/TOF MS) method was developed for the separation and identification of bovine lactoferricin(LfcinB).Bovine lactoferrin was hydrolyzed by pepsin and then separated by ion exchange chromatography and reversed-phase liquid chromatography(RP-LC).The antibacterial activities of the fractions from RP-LC separation were determined and the protein concentration of the fr... |
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| Keywords: | high performance liquid chromatography (HPLC) matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS) bovine lactoferricin bovine lactoferrin |
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