Determination of baicalin, chlorogenic acid and forsythin in Chinese medicinal preparation by capillary electrophoresis |
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Authors: | Xiangjun Li Yuping Zhang Zhuobin Yuan |
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Institution: | (1) Department of Chemistry, Graduate School, USTC, Chinese Academy of Sciences, 100039 Beijing, China |
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Abstract: | Summary The determination of baicalin, chlorogenic acid and forsythin in a traditional Chinese medicinal preparation, Shuanghuanglian
oral liquid, has been investigated by micellar electrokinetic capillary electrophoresis using borate buffer, SDS and acetonitrile
as background electrolyte, 15 kV applied voltage and UV detection, the three active compounds were completely separated within
15 min. The effect of buffer pH, concentration of borate and SDS are discussed. Regression equations reveal linear relationships
between the peak-area of each component and content. In addition, the levels of three active compounds in traditional Chinese
medicinal preparation, Shuanghuanglian oral liquid, were easily determined with recoveries of 95.20–105.73%. |
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Keywords: | Capillary electrophoresis Chinese herbal medicine Baicalin chlorogenic acid and forsythin |
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