A Fluorescence Quenching Method for the Determination of Nitrite with Indole |
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| Authors: | Nianqin Jie Duanling Yang Quibin Jiang Qiang Zhang Lu Wei |
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| Affiliation: | a Department of Chemistry, Shandong University, Jinan, People's Republic of China;b Reserch Center for Eco-Environmental Science, Chinese Academy of Science, Beijing, People's Republic of China;c Institute of Science and Technology Information of China, Beijing, People's Republic of China |
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| Abstract: | A new fluorescence quenching method has been developed to determine nitrite in water and food samples. The method is based on the reaction between nitrite and the fluorescent indole to form a compound which has no fluorescence in acidic medium. The fluorescence intensity was measured in 1 cm quartz cell with excitation and emission wavelengths of 285 and 350 nm, respectively. The relationship was obtained between the fluorescence intensity and nitrite concentration in the range 0.01–0.6 μg ml−1. The detection limit was 2.5 ng ml−1. The proposed method was applied to determine nitrite in water and food samples. The mechanism involved in the reaction was studied. |
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| Keywords: | nitrite indole fluorimetry |
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