尿中核苷排放模式测定的两种方法─-HPLC和CE法(英文)

癌症病人在尿中排放比常人高得多的叶啉和嘧啶，这些改性核耷是RNA的主要组成。由于其增加的排放与癌变的RNA周转有关，已被建议和研究作肿瘤标记物。文章给出测定尿中核苷的反相高效液相色谱法和毛细管电泳方法。两种方法所得数据一致。用此方法建立了正常人尿中核苷的排放水平，并测定了34个癌症病人尿中核苷浓度，观察到改性核着浓度明显的增高现象。结果袭明，两个方法均适于大量尿样的核苷与癌症关系的临床研究。

Two Approaches for Determining the Urinary Excretion Patterns of Nucleosides-HPLC and CE

Cancer patients excrete in their urine increased amounts of modified purines and pyrimidines. These modified mucleosides are primary constitutents of RNA and are synthesized at the macromolecular level. When RNA is catabolized, most of these modified nucleosides cannot be reutilized ; consequently, they are excreted. Due to their increased urinary excretion in conjunction with altered RNA turnover in carcinogenesis, they have been proPO8ed as tumour markers. We developed both reversed-phase high performance liquid chromatographic and capillary electrophoretic approaches to determine normal and modified nucleosides in urine. Data obtained with CE are in good accordance with those from HPLC. The reference excretion levels of urinary nucleosides from healthy volunteers were established, and elevated ones of modified nucleosides from 34 patients with different kinds of cancer were observed. The developed methods are suitable for the analysis of large series of samples for clinical studies.