液相色谱-串联质谱法测定食品中合成色素

提出了液相色谱-串联质谱法测定食品中8种合成色素柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝、赤藓红和偶氮玉红的含量。固体(半固体)样品经乙醇-氨水-水(70+1+29)溶液溶解,所得滤液烘干后用水溶解,经Waters Atlantis dC18色谱柱(2.1 mm×150 mm,5μm)分离,用甲醇和10 mmol.L-1乙酸铵溶液以不同体积比混合进行梯度洗脱,采用电喷雾正离子模式串联质谱检测。8种合成色素的质量浓度均在50μg.L-1以内与其峰面积呈线性关系,检出限(3S/N)在0.003～0.020 mg.kg-1之间。方法应用于食品样品中8种合成色素的测定,回收率在83.0%～112.0%之间,测定值的相对标准偏差(n=6)均小于5%。

LC-MS/MS Determination of Synthetic Colorants in Food

LC-MS/MS was applied to the determination of 8 kinds of synthetic colorants,including tartrazinein,amaranth,ponceau 4R,sunset yellow,fancy red,EDS,food red 14 and azorubine in food.The solid or semi-solid samples were dissolved with C2H5OH-NH3(aq.) and H2O(70+1+29) solution,and the filtrate liquor was evaporated to dryness.The residue was dissolved with water and used for LC-MS/MS determination,using Waters Atlantis dC18 column(2.1 mm×150 mm,5 μm) as chromatographic column,and a mixture of methanol and 10 mmol·L-1 ammonium acetate with the different ratios as mobile phase for gradient elution.Positive electrospray ionization mode was used in the MS/MS detection.Linear relationships between values of peak area and mass concentration of the 8 synthetic colorants were obtained in the range within 50 μg·L-1 with their detection limits(3S/N) in the range of 0.003-0.020 mg·kg-1.The proposed method was used to the determination of the 8 synthetic colorants in food,giving values of recovery in the range of 83.0%-112.0% with RSD′s(n=6) less than 5%.