HILIC mode separation of polar compounds by monolithic silica capillary columns coated with polyacrylamide |
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Authors: | Tohru Ikegami Hiroshi Fujita Kanta Horie Ken Hosoya Nobuo Tanaka |
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Institution: | (1) Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan |
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Abstract: | HILIC mode columns were prepared by an on-column polymerization of acrylamide on a monolithic silica capillary column modified
with N-(3-trimethoxysilylpropyl)methacrylamide as the anchor group. The products showed HILIC mode retention characteristics with
three times greater permeability and slightly higher column efficiency compared to a commercially available amide-type HILIC
column packed with 5-μm particles. The selectivity of the monolithic silica-based column was similar to that of the particulate
column for each group of solutes towards nucleosides, nucleic bases and carbohydrate derivatives, although a considerable
difference was observed in the selectivity for the solute groups. Although the retention of solutes based on the polar functionality
was much smaller with the monolithic silica columns, which had a smaller phase ratio, than with the particle-packed column,
the former can achieve better separation utilizing the high permeability and higher column efficiencies of a longer column. |
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Keywords: | Monolithic silica HILIC Polyacrylamide PA sugars Nucleosides |
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