| Determination of selectivity of HPLC systems by correspondence factor analysis |
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| 引用本文： | Yuan Wang Jun Yang Xin Lu Guo Wang Xu. Determination of selectivity of HPLC systems by correspondence factor analysis[J]. 中国化学快报, 2007, 18(5): 565-568. DOI: 10.1016/j.cclet.2007.03.026 |
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| 作者姓名： | Yuan Wang Jun Yang Xin Lu Guo Wang Xu |
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| 作者单位： | National Chromatographic Research ＆ Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
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| 基金项目： | 国家自然科学基金;国家自然科学基金;中国科学院知识创新工程项目 |
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| 摘 要： | Correspondence factor analysis （CFA） was employed to study the selectivity of 14 HPLC systems. The tested LC systems were classified as reversed-phase （RP）, ion-exchange （IE） and hydrophilic interaction chromatography （HILIC） modes. It was found that the retentions of the hydrophilic solutes on HILIC column were significantly influenced by the second-order effects besides their hydrophilic properties. Organic modifiers and residue silanol groups on silica surface both participated in retention. HypersilTM amino column performed separation in the HILIC mode at appropriate conditions, and its retention mechanism was more similar to that of HILIC silica column than that of HILIC column coating poly（aspartamide） groups.
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| 关 键 词： | HPLC系统 测定 选择性 对应因子分析 |
| 收稿时间： | 2006-12-31 |
Determination of selectivity of HPLC systems by correspondence factor analysis |
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| Yuan Wang,Jun Yang,Xin Lu,Guo Wang Xu. Determination of selectivity of HPLC systems by correspondence factor analysis[J]. Chinese Chemical Letters, 2007, 18(5): 565-568. DOI: 10.1016/j.cclet.2007.03.026 |
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| Authors: | Yuan Wang Jun Yang Xin Lu Guo Wang Xu |
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| Affiliation: | National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
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| Abstract: | Correspondence factor analysis (CFA) was employed to study the selectivity of 14 HPLC systems. The tested LC systems were classified as reversed-phase (RP), ion-exchange (IE) and hydrophilic interaction chromatography (HILIC) modes. It was found that the retentions of the hydrophilic solutes on HILIC column were significantly influenced by the second-order effects besides their hydrophilic properties. Organic modifiers and residue silanol groups on silica surface both participated in retention. Hypersil™ amino column performed separation in the HILIC mode at appropriate conditions, and its retention mechanism was more similar to that of HILIC silica column than that of HILIC column coating poly(aspartamide) groups. |
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| Keywords: | CFA HILIC Liquid chromatography |
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