| Monitoring the Hydrolysis of p—Nitrophenyl Acetate Catalyzed by Seryl—histidine with Electrospray Ionization Mass Spectrometry |
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| 引用本文： | 陈晶,张韵,曹晓宇,王津,陈益,赵玉芬. Monitoring the Hydrolysis of p—Nitrophenyl Acetate Catalyzed by Seryl—histidine with Electrospray Ionization Mass Spectrometry[J]. 中国化学, 2002, 20(10): 1097-1101. DOI: 10.1002/cjoc.20020201031 |
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| 作者姓名： | 陈晶 张韵 曹晓宇 王津 陈益 赵玉芬 |
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| 作者单位： | CHEN，Jing a ZHANG，Yun b CAO，Xiao-Yu b WANG，Jin b CHEN，Yi a ZHAO，Yu-Fen aKey Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry，Department of Chemistry，School of Life Sciences and Engineering，Tsinghua Universit |
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| 基金项目： | ProjectsupportedbytheChineseNationalNaturalScienceFoundation (Nos .2 96 32 0 0 4and 39870 415 )ofChina |
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| 摘 要： | ＩｎｔｒｏｄｕｃｔｉｏｎＩｎｔｈｅｐａｓｔｄｅｃａｄｅ ,ｉｔｗａｓｆｏｕｎｄｔｈａｔｔｗｏａｍｉｎｏａｃｉｄｒｅｓｉｄｕｅｓ ,ＳｅｒａｎｄＨｉｓ ,ｗｏｒｋａｓｔｈｅａｃｔｉｖｅｓｉｔｅｓｉｎｔｈｅｓｅｒ ｉｎｅｐｒｏｔｅａｓｅ .1 3 Ｉｎｏｕｒｐｒｅｖｉｏｕｓｗｏｒｋ ,ａｄｉｐｅｐｔｉｄｅｓｅｒｙｌ ｈｉｓｔｉｄｉｎｅ (Ｓｅｒ Ｈｉｓ)ｗａｓｆｏｕｎｄｔｏｈａｖｅｔｈｅｐｒｏｔｅｉｎａｎｄｎｕ ｃｌｅｏｓｉｄｅｓｃｌｅａｖａｇｅａｃｔｉｖｉｔｙ .4 7ｐ Ｎｉｔｒｏｐｈｅｎｙｌａｃｅｔａｔｅ (ｐ ＮＰＡ)ｗ…
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| 关 键 词： | 硝基苯醋酸盐 丝氨酰－组氨酸 静电离子化作用 光谱法 催化合成 |
Monitoring the Hydrolysis of p-Nitrophenyl Acetate Catalyzed by Seryl-histidine with Electrospray Ionization Mass Spectrometry |
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| CHEN,Jing a ZHANG,Yun b CAO,Xiao-Yu b WANG,Jin b CHEN,Yi a ZHAO,Yu-Fen aKey Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry. Monitoring the Hydrolysis of p-Nitrophenyl Acetate Catalyzed by Seryl-histidine with Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Chemistry, 2002, 20(10): 1097-1101. DOI: 10.1002/cjoc.20020201031 |
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| Authors: | CHEN Jing a ZHANG Yun b CAO Xiao-Yu b WANG Jin b CHEN Yi a ZHAO Yu-Fen aKey Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry |
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| Affiliation: | CHEN,Jing a ZHANG,Yun b CAO,Xiao-Yu b WANG,Jin b CHEN,Yi a ZHAO,Yu-Fen aKey Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry,Department of Chemistry,School of Life Sciences and Engineering,Tsinghua Universit |
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| Abstract: | The hydrolysis of p‐nitrophenyl acetate (p‐NPA) catalyzed by seryl‐histidine or histidine has been monitored by electrospray ionization mass spectrometry in the presence of the internal calibration, 8‐anilino‐1‐naphthalenesulfonic acid ammonium salt (ANS). The half‐life of p‐NPA in the presence of 10 mmol·L?1 seryl‐histidine or histidine at 25 °C was 370 min and 70 min respectively. With the occurrence of acetyl seryl‐histidine and acetyl histidine in the reaction, and the fact that p‐NPA was stable in the presence of 10 mmol·L?1 serine, an imidazolysis mechanism has been proposed, which is in accordance with the reported work. |
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| Keywords: | reaction monitoring hydrolysis p-nitrophenyl acetate ESI-MS |
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