A Flow System Based on a Fluorometer and a Luminometer to Monitor the Correlation of Protein Conformation and Function |
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| Authors: | T Ikkai K Shimada |
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| Institution: | (1) Aichi Prefectural University of Fine Arts, Nagakute, Aichi, 480-1194, Japan;(2) Visual and Urvan Design, School of Design and Architecture, Nagoya City University, Chikusa-ku, Nagoya City, 464-0083, Japan |
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| Abstract: | A flow system to monitor the reactions in solutions was constructed, which connected a fluorometer and a luminometer in series. Using a stream through it, the correlation of fluorescence and luminescence was measured. The system was tested with actin. The fluorescence emission gave the signal of polymerization of labeled actin and the luminescence gave that of ATP hydrolysis by actin. The observations showed the correlation between ATP hydrolysis and polymerization. Applications of other proteins to this system will give insights into the characteristics of proteins. |
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| Keywords: | Flow excimer emission luminescence actin ATPase |
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