Spectrofluorimetric Determination of Famotidine in Pharmaceutical Preparations and Biological Fluids. Application to Stability Studies |
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Authors: | M I Walash A El-Brashy N El-Enany M E Kamel |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt |
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Abstract: | A simple, economic, selective, and stability indicating spectrofluorimetric method was developed for the determination of
famotidine (FMT); is based on its reaction with 9, 10-phenanthraquinone in alkaline medium to give a highly fluorescent derivative
measured at 560 nm after excitation at 283 nm. The fluorescence intensity - concentration plot was rectilinear over the concentration
range of 50–600 ng/ml with minimum quantification limit (LOQ) of 13.0 ng/ml and minimum detection limit (LOD) of 4.3 ng/ml.
The factors affecting the development of the fluorescence intensity of the reaction product were carefully studied and optimized.
The method was applied for the determination of FMT in its dosage forms. The stability of the compound was studied, and the
proposed method was found to be stability indicating one. The results obtained were in good agreement with those obtained
by the official method. Furthermore, the method was applied for the determination of FMT in spiked and real human plasma.
The mean % recovery (n = 4) was found to be 99.94 ± 0.24, and 105.13 ± 0.64 for spiked and real human plasma, respectively. The composition of the
reaction product as well as its stability constant was also investigated. Moreover, the method was utilized to investigate
the kinetics of both alkaline and oxidative induced degradation of the drug. The apparent first order rate constant and half
life time of the degradation product was calculated. A proposal of the reaction pathway was postulated. |
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Keywords: | Famotidine Spectrofluorimetry 9 10-Phenanthraquinone |
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