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Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics |
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| Authors: | Dr. Linda Cerofolini Stefano Giuntini Dr. Azzurra Carlon Dr. Enrico Ravera Dr. Vito Calderone Prof. Marco Fragai Prof. Giacomo Parigi Prof. Claudio Luchinat |
| Affiliation: | Magnetic Resonance Center (CERM), University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di, Metallo Proteine (CIRMMP), Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy |
| Abstract: | Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to “evade” the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography. |
| Keywords: | biopharmaceuticals PEGylation protein modifications protein structures structural biology |