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Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins |
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| Authors: | Dongxiang Xue Tiandan Xu Huixia Wang Meng Wu Ya Yuan Wei Wang Qiwen Tan Dr Fei Zhao Fang Zhou Tao Hu Prof Zhongxing Jiang Prof Zhi-Jie Liu Prof Suwen Zhao Prof Dongsheng Liu Prof Kurt Wüthrich Prof Houchao Tao |
| Institution: | 1. iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210 China
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai, 201203 China University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049 China School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 China These authors contributed equally to this work.;2. iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210 China University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049 China School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 China CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031 China These authors contributed equally to this work.;3. iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210 China University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049 China School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 China CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031 China;4. iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210 China;5. Hubei Province Engineering and Technology Research Center, for Fluorinated Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071 China;6. iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210 China Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai, 201203 China University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing, 100049 China School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 China;7. iHuman Institute, ShanghaiTech University, Ren Building, 393 Middle Huaxia Road, Pudong New District, Shanghai, 201210 China School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 China |
| Abstract: | Disulfide-containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein-coupled receptor A2AAR, which is an α-helical protein, and the β-barrel protein OmpX from E. coli. |
| Keywords: | detergents disulfides membrane proteins micelle size NMR spectroscopy |