Metabolic Studies of Tumor Cells Using [1-13C] Pyruvate Hyperpolarized by Means of PHIP-Side Arm Hydrogenation |
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Authors: | Dr Eleonora Cavallari Dr Carla Carrera Prof Silvio Aime Dr Francesca Reineri |
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Institution: | Dept. Molecular Biotechnology and Health Sciences, University of Torino, Via Nizza 52, Torino, Italy |
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Abstract: | The kinetics of metabolic processes can be assessed, in real time by means of MR hyperpolarized (HP) metabolites. 1-13C]pyruvate, hyperpolarized by means of d-DNP, is, by far, the substrate most widely applied to the investigation of several pathologies characterized by deregulated glycolytic metabolic networks, including cancer. Hyperpolarization of 1-13C]pyruvate by means of the cost effective, fast and easy to handle PHIP-SAH (para-hydrogen induced polarization-side arm hydrogenation) method opens-up a pathway for the application of HP metabolites to a wide range of cancer-related studies. Herein, we report the first application of PHIP-SAH hyperpolarized 1-13C]pyruvate in the investigation of upregulated glycolysis in two murine breast cancer cell lines (168FARN and 4T1). The results obtained using HP pyruvate have been validated with a conventional biochemical assay and are coherent with previously-reported lactate dehydrogenase activity measured in those cells. |
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Keywords: | Cancer hyperpolarization metabolism NMR spectroscopy parahydrogen |
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