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Mitochondria Targeting Fluorescent Probes Based on through Bond-Energy Transfer for Mutually Imaging Signaling Molecules H2S and H2O2 |
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| Authors: | Dr Jian Sun Xiao Li Jia Cao Prof Qi Sun Dr Yajie Zhang Xuewei Wang Tiantian Wu Xiantao Hu Prof Fude Feng |
| Institution: | 1. Department of Polymer Science & Engineering, Nanjing University, Jiangsu, Nanjing, 210023 P. R. China;2. Key Laboratory of Mesoscopic Chemistry of Ministry of Education, Institute of Theoretical and Computational Chemistry, Nanjing University, Jiangsu, Nanjing, 210023 P. R. China;3. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Jiangxi, Nanchang, 330013 P. R. China |
| Abstract: | Reactive signaling molecules participate in varieties of biochemical reactions, and methods to detect their mutual existence and crosstalk are in urgent demand. A benzothiadiazole-based handle was designed to fluorescently respond to the co-existence of H2S and H2O2 under pseudo-physiological conditions on a basis of a thiyl-radical-mediated mechanism that accounts for the rapid and efficient domino-like reaction processes. Then the handle motif was attached to a rhodamine moiety by means of an ethynylene linkage, and achieved a significant H2S–H2O2 mutual response in the mitochondria of living cells. Theoretical calculations supported that a through bond energy transfer mechanism contributes to the drastic fluorescence response. |
| Keywords: | cell imaging energy transfer hydrogen sulfide mitochondria reactive oxygen species |