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Direct Measurement of Through-Bond Effects in Molecular Multivalent Interactions |
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| Authors: | Xueyan Feng Chanchan Yu Feng Feng Pan Lu Yahong Chai Qilong Li Dr Di Zhang Dr Xiuyu Wang Prof Li Yao |
| Institution: | 1. Beijing National Laboratory for Molecular Science, Institute of Chemistry Chinese Academy of Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing, 100190 P. R. China
University of Chinese Academy of Science, Beijing, 100049 P. R. China;2. Beijing National Laboratory for Molecular Science, Institute of Chemistry Chinese Academy of Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing, 100190 P. R. China |
| Abstract: | Multivalent interactions occur throughout biology, and have a number of characteristics that monovalent interactions do not. However, it remains challenging to directly measure the binding force of molecular multivalent interactions and identify the mechanism of interactions. In this study, the specific interaction between bivalent aptamer and thrombin has been measured directly and quantitatively by force-induced remnant magnetization spectroscopy to investigate the binding force and through-bond effects of the multivalent interactions. The measured differential binding forces enable through-bond effects in thrombin–aptamer complexes to be identified, where aptamer binding at exosite II produces visible effects on their binding at exosite I and vice versa. This method might be suitable for practical applications in the design of high-performance ligands. |
| Keywords: | aptamers force-induced remnant magnetization spectroscopy multivalent interactions thrombin through-bond effects |