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Oligopeptide Helical Conformations Control Gold Nanoparticle Cross-Linking
Authors:Yanchao Lyu  Dr Giulia Marafon  Dr Álvaro Martínez  Prof Dr Alessandro Moretto  Prof Dr Paolo Scrimin
Institution:1. Department of Chemical Sciences, University of Padova, Via Marzolo, 1, 35131 Padova, Italy;2. Department of Chemical Sciences, University of Padova, Via Marzolo, 1, 35131 Padova, Italy

Current address: International Physics Center, Paseo Manuel de Lardizabal 4, Donostia, 20018 Spain

Abstract:Peptide sequences functionalized with primary amines at the N- and C-terminus are able to induce the aggregation of gold nanoparticles in ethanol as a consequence of their folding into a helical conformation. Random coil peptides are unable to induce such an aggregation process. Aggregation can be monitored spectrophotometrically by following the shift of the surface plasmon resonance (SPR) band of the nanoparticles and is confirmed by transmission electron microscopy and dynamic light scattering analyses. Partial denaturation of the peptides results in diminished cross-linking ability. The helicity parameter θ222/θ208 correlates fairly well with the shift of the SPR band to longer wavelengths, supporting the relationship between the amount of helical content of a peptide sequence and its ability to induce aggregation.
Keywords:Au–N interactions  cross-linking  gold nanoparticles  helical peptides  plasmon resonance band
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