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Single-Sided Competitive Axial Coordination of G-Quadruplex/Hemin as Molecular Switch for Imaging Intracellular Nitric Oxide |
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| Authors: | Dr Lei Zhang Dr Jun Zhou Fengjiao Ma Dr Quanbo Wang Prof Hui Xu Prof Huangxian Ju Prof Jianping Lei |
| Institution: | 1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P.R. China
School of Chemistry and Molecular Engineering, Institute of, Advanced Synthesis, Jiangsu National Synergetic Innovation Center for, Advanced Materials, Nanjing Tech University, Nanjing, 211816 P.R. China These authors contributed equally to this work.;2. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P.R. China These authors contributed equally to this work.;3. Laboratory of Immunology for Environment and Health, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, 250014 P.R. China;4. School of Chemistry and Molecular Engineering, Institute of, Advanced Synthesis, Jiangsu National Synergetic Innovation Center for, Advanced Materials, Nanjing Tech University, Nanjing, 211816 P.R. China;5. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P.R. China |
| Abstract: | Axial coordination is a crucial biological process to regulate biomolecules’ functions in natural enzymes. However, it is a great challenge to determine the single or dual axial interaction between the metal center of enzymes and the ligand. In this work, a controllable axial coordination system was developed based on G-quadruplex/hemin complex by designing a series of fluorescent derivatives. The mechanism on axial coordination of G-quadruplex/hemin with coumarin-imidazole ligands was proposed to be single-sided, and led to fluorescence quenching of ligands. Upon addition of nitric oxide, the fluorescence of ligands was recovered through competitive axial coordination, providing a “signal on” strategy for signal transduction. More significantly, the fluorescent imaging of intracellular nitric oxide was achieved after conjugating with gold nanoparticles. Also, the proposed protocol provided a smart strategy to monitor the relationship between nitric oxide and p53 protein activity in living cells. |
| Keywords: | axial coordination biosensors fluorescent probes G-quadruplex-hemin imaging |