Determination of tertatolol enantiomers in biological fluids by high-performance liquid chromatography. |
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Authors: | T Lave C Efthymiopoulos J C Koffel L Jung |
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Affiliation: | Faculté de Pharmacie, Laboratoire de Pharmacie Chimique, Illkirch-Graffenstaden, France. |
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Abstract: | A stereospecific high-performance liquid chromatographic method for the quantification of (-)- and (+)-tertatolol in plasma and urine is described. The method involves solid-phase extraction followed by derivatization with S(+)-naphthylethylisocyanate to form the urea derivative, which is more sensitive to fluorescence detection. The separation of the diastereomeric derivatives was performed by reversed-phase high-performance liquid chromatography. Fluorimetric detection (lambda excitation = 220 nm, lambda emission = 320 nm) allows the quantification of tertatolol enantiomers down to 6 ng/ml. The assay was used to study the pharmacokinetic profile of tertatolol enantiomers following oral administration of racemic tertatolol; preliminary results suggest enantioselective absorption and/or disposition of tertatolol. |
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