Comparative study of inorganic elements determination in whole blood from Crioula breed horse by EDXRF and NAA analytical techniques |
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Authors: | Tatyana S Baptista Marcelo M Redígolo Cibele B Zamboni Ivone M Sato Jose R Marcelino |
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Institution: | 1.Instituto de Pesquisas Energéticas e Nucleares - Centro de Reatores de Pesquisa,Av. Prof. Lineu Prestes,2242 – Cidade Universitária,S?o Paulo,Brazil;2.Instituto de Pesquisas Energéticas e Nucleares - Centro de Química e Meio Ambiente,Av. Prof. Lineu Prestes, 2242 – Cidade Universitária,S?o Paulo,Brazil;3.Instituto Butantan - Se??o de Processamento de Plasmas Hiperimunes.Av Vital Brasil,S?o Paulo,Brazil |
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Abstract: | The World Health Organization states that envenomation is responsible for a high number of deaths per year, especially in
equatorial areas. The only effective specific treatment is the use of hyperimmune serum (antivenom). In Brazil, Crioula breed
horses are used for antivenom production, with great importance in the maintenance of public health programs. A strict biochemical
and metabolic control is required to attain specificity in antiserum. Inorganic elements represent only a small fraction of
whole blood. Nonetheless, they play important roles in mammalian metabolism, being responsible for controlling enzymatic reactions,
respiratory and cardiac functions and ageing. In this work, whole blood samples from Crioula breed horses were analyzed by
EDXRF technique. The reference interval values were determined for the elements Na (1955–2013 μg g−1), Mg (51–75 μg g−1), P (523–555 μg g−1), S (1628–1730 μg g−1), Cl (2388–2574 μg g−1), K (1649–1852 μg g−1), Ca (202–213 μg g−1), Cu (4.1–4.5 μg g−1) and Zn (2.4–2.8 μg g−1) and a comparative study with NAA results was outlined. The samples were obtained from Instituto Butantan. Both techniques
showed to be appropriate for whole blood sample analyses and offer a new perspective in Veterinary Medicine. |
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