Quantitation of nifedipine in human plasma by on-line solid-phase extraction and high-performance liquid chromatography |
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Authors: | Yriti M Parra P Iglesias E Barbanoj J M |
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Institution: | Analysis Laboratory, Research Institute, Sant Pau Hospital, Barcelona, Spain. ifarm@santpau.es |
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Abstract: | An analytical methodology for nifedipine quantitation in plasma by on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contain C2 and the analytes nifedipine and nitrendipine (internal standard) are separated on a C18 column with a mobile phase consisting of acetonitrile–13 mM phosphate buffer pH 7 (65:35, v/v) followed by UV detection at 338 nm. Validation of the method demonstrated good recoveries (>90%), sensitivity (limit of quantification, 2 ng/ml), based on a 500 μl sample volume, accuracy and precision (<5.5% in concentrations greater than the limit of quantitation). This methodology has been used for bioequivalence studies. |
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Keywords: | Nifedipine Nitrendipine |
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