Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed |
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Authors: | Feng Wang Chen Guo Chun-Zhao Liu |
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Institution: | 1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China
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Abstract: | A magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes versicolor. The MMSNPs-Cu2+ particles in the MSFB maintained a stable bed expansion of two to threefold at a flow rate of 120–180 cm/h. At the optimal magnetic field intensity of 120 Gs, both the maximal Bodenstein number and the smallest axial dispersion coefficient were achieved, which resulted in a stable fluidization stage. The dynamic binding capacity of laccase in the MSFB decreased from 192.5 to144.3 mg/g when the flow velocity through the bed increased from 44.2 to 69.8 cm/h. The MSFB with MMSNPs-Cu2+ achieved efficient laccase purification from the fermentation broth with 62.4-fold purification of laccase and 108.9 % activity yield. These results provided an excellent platform for the application of these magnetic mesoporous nanoparticles integrated with the MSFB in developing novel protein purification process. |
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