Synthesis and lectin-binding activity of luminescent silica particles peripherally functionalized with lactose |
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Authors: | Ken Hatano Tetsuya Yamazaki Koji Yoshino Naoto Ohyama Tetsuo Koyama Koji Matsuoka Daiyo Terunuma |
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Affiliation: | Division of Material Science, Graduate School of Science and Technology, Saitama University, 255 Shimo-Ohkubo, Sakura-ku, Saitama 338-8570, Japan |
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Abstract: | A novel O-protected lactose (Galβ1→4Glcβ1-) derivative bearing trimethoxysilyl group at the aglycon was developed as a silane coupling agent. Reaction of the coupling agent with tris(2,2′-bipyridine)ruthenium (II) dichloride (Rubpy) doped silica particle gave a Rubpy-doped silica particle peripherally functionalized with O-protected lactose derivative. De-O-protection of the particle with aqueous ammonia provided lactose-coating Rubpy-doped silica particles, combining luminophor encapsulated in silica matrix and carbohydrate having lectin-recognition ability. Specific adhesion of fluorescein isothiocyanate-labeled peanut agglutinin (FITC-PNA) to the lactose-coating Rubpy-doped silica particles was confirmed by fluorescence microscopic analysis. |
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Keywords: | Carbohydrate Glycocluster Rubpy Silica particles Lectin-binding Luminescent scaffold Silane coupling agent |
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