A novel method for D-arabinitol determination based on a nano-structured sensing film by one-step electrodeposition |
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Authors: | Fang Wang Yaqian Liao Yi Ren Zilin Chen |
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Institution: | 1. Department of Analytical Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Stanislawa Wyspianskiego 27, 50-370, Wroclaw, Poland 2. Department of Biotechnology, Faculty of Biological Sciences, University of Zielona Gora, Prof. Z. Szafrana 1, 65-516, Zielona Gora, Poland
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Abstract: | Copper nanoparticles (Cu-NPs) were incorporated into chitosan hydrogel to form a film on the surface of a glassy carbon electrode (GCE) leading to a sensing element for D-arabinitol with excellent oxidative catalytic activity. The electrochemical response to D-arabinitol was studied by cyclic voltammetry and differential pulse voltammetry. Operational parameters affecting the response were examined and optimized, and a simple and sensitive method was established for the determination of D-arabinitol. Response is linear in the concentration range from 10 μmol·L?1 to 10 mmol·L?1, and the limit of detection is 1.0 μmol·L?1. The method may be combined with separation techniques in order to analyze for the ratio of D- and L-arabinitol which is a diagnostic marker for candidiasis. |
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